Systematic Review · e0004

Circulating Tumor DNA for Molecular Residual Disease Detection and Multicancer Early Detection: A Systematic Review

Citation

A.Sh. Ibrayeva, B.S. Asembekov, L.N. Ibragimova, M.M. Maemerov, D.K. Davletov, K.D. Kovaleva. Circulating Tumor DNA for Molecular Residual Disease Detection and Multicancer Early Detection: A Systematic Review. Global Medical Reviews. 2026;1(1):e0004.

Abstract

Background

Circulating tumor DNA is clinically advanced in oncology, but its value differs between molecular residual disease assessment after curative-intent treatment and multicancer early detection in asymptomatic populations.

Objective

To evaluate clinical effectiveness, actionability, and certainty of evidence for ctDNA-based MRD strategies and cfDNA- or ctDNA-based MCED pathways.

Methods

The review followed PRISMA 2020 and PRISMA-S. Databases and trial registries were searched through July 2026. Two reviewers independently screened records, extracted data, and assessed risk of bias.

Results

Twenty-nine completed reports representing 25 study programs were included. Selected MRD trials demonstrated clinical utility when test results were linked to defined treatment decisions.

Conclusions

ctDNA has demonstrated clinical utility in selected MRD settings. In many other tumors it remains primarily prognostic, and broad MCED implementation is premature.

Keywords

circulating tumor DNActDNAliquid biopsymolecular residual diseasemulticancer early detectionMCED